N2910
NmuCI (Tsp45I)
Size
£364.00
- SKU:
- N2910
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Extra Details:
- C 5'-^G T G A C-3' 3'-C A C T G^-5' G Concentration: 10u/ul Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C. Diluent Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with NmuCI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with NmuCI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.8uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Methylation Effects: GT(C/G)m5C is cleaved by NmuCI at a significantly slower rate. Overlapping CG methylation may influence DNA cleavage. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Compatible Ends: Eco91I (G^GTCACC) MaeIII (^GTGAC). Number of Recognition Sites in DNA: Lambda: 81 PhiX174: 8 M13mp18/19: 8 pBR322: 9 pUC18/19: 4 pUC57: 4 pTZ19R/U: 4 pBluescriptIIKS(-/+): 4 pBluescriptIISK(-/+): 4 pACYC177: 5 pACYC184: 7 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
- Shipping Conditions:
- Blue Ice