N2904-35

NMDA 1 Receptor (NMDA R1, N-methyl-D-aspartate)

Size

£863.00

SKU:: N2904-35
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant fusion protein containing GST and NMDAR1 (aa 660-811). Epitope: extracellular loop between transmembrane regions III and IV.
Clone:: 2Q187 (54.1)
Format:: Supplied as a liquid in PBS, pH 7.4. No preservative added.
Extra Details:: The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl- 4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience. They are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors appear to be a target for ethanol at physiological concentrations which may play a significant role in alcoholism. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Light and Electron Microscopy, ELISA and RIA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:200. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Use standard deparaffinization techniques on tissue specimens. Pretreat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Do not allow tissues to dry out during the staining procedure. Immunohistochemistry (Frozen): rat spinal cord Optimal dilution determined by the researcher. Immunohistochemistry Control: Rat brain and spinal cord tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice