N2904-31
NMDA 1 Receptor (NMDA R1, N-methyl-D-aspartate, N-methyl-D-aspartate Receptor Channel, subunit zeta-1 NR1, Glutamate [NMDA] Receptor subunit zeta 1, Ionotropic, N-methyl D-aspartate 1)
Size
£863.00
- SKU:
- N2904-31
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa909-938, LQNQKDTVLPRRAIEREEGQLQLCSRHRES, from the C-terminus of rat NMDA receptor subunit (Accession# NM_000832.5). Species Sequence Homology: monkey, gerbil, rabbit, feline and fish.
- Clone:
- 9L865 (1.17.2.6)
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Extra Details:
- The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4- isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detects NMDAR1 on 10ug of rat brain lysate. Labels a single band at ~120kD corresponding to NMDAR1 in rat brain lysate. Immunohistochemistry: 1:1000-1:2000 with light microscopy. Tested with 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Control: Brain tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice