N2851
Nkx 5.1 (NK-2 Family Homeodomain Transcription Factor)
Size
£825.00
- SKU:
- N2851
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa311-328 of human Nkx5.1
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- Applications: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:200-1:1000 Immunocytochemistry: 1:200-1:1000 Western Blot (ECL): 1:200-1:1000 Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat -10um slices 3. Dry slides at RT for 30-90 minutes 4. Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS 5. Let air dry 6. PBS 5 minutes 7. Incubate in 50mM ammonium chloride 30 minutes 8. PBS 5 minutes 9. Blocking serum 30-45 minutes 10. N2851 90 minutes - dilution 1:200 - 1:1000 11. Wash 3 times for 5 minutes 12. Secondary fluorescent conjug. antibody 30 minutes 13. Wash 3 times for 5 minutes and mount Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice