Nkx 5.1 (NK-2 Family Homeodomain Transcription Factor) (Biotin)

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£1,070.00

SKU:: N2851-BIOTIN
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa311-328 of human Nkx5.1
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: 1. Dissect tissues and freeze on dry ice 2. Cut on a cryostat -10um slices 3. Dry slides at RT for 30-90 minutes 4. Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS 5. Let air dry 6. PBS 5 minutes 7. Incubate in 50mM ammonium chloride 30 minutes 8. PBS 5 minutes 9. Blocking serum 30-45 minutes 10. N2851 90 minutes - dilution 1:200 - 1:1000 11. Wash 3 times for 5 minutes 12. Secondary fluorescent conjug. antibody 30 minutes 13. Wash 3 times for 5 minutes and mount Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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