N2851-75H
NKG2A/C/E (Killer Cell Lectin-like Receptor Subfamily C, Member 1/2/3, Natural Killer Cell Protein Group 2-A1, Natural Killer Cell Protein Group 2-C1, Natural Killer Cell Receptor NKG2A, Natural Killer Cell Receptor NKG2C, Natural Killer Cell Group 2E Cell Receptor NKG2E)
Size
£800.00
- SKU:
- N2851-75H
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Stable Chinese hamster ovary (CHO) cells expressing the B6 alleles of CD94 and NKG2A.
- Clone:
- 20D5
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- Killer cell lectin-like receptor subfamily C, member 1/2/3 (UniProt: Q9Z202/ Q9WTJ9/Q9QXN7; also known as Natural killer cell protein group 2-A1/ Natural killer cell protein group 2-C1, Natural killer cell receptor NKG2A/Natural killer cell receptor NKG2C/ Natural killer cell group 2E cell receptor NKG2E) are encoded by the Klrc1/2/3 genes (Gene IDs: 16641/ 16642/58179) in murine species. The NKG2 proteins are members of the lectin-like receptor family that heterodimerize with CD94 on the surface of NK and NKT cells. They are also shown to be expressed on activated CD8+ T cells. They are integral component of membrane and are present on the external side of plasma membrane. Within their extracellular carbohydrate recognition domains, NKG2A, NKG2C and NKG2E share extensive homology; however, NKG2C and NKG2E receptors differ significantly from NKG2A in their cytoplasmic domains. NKG2/CD94 heterodimeric complexes are shown to recognize Qa-1 that is described as a nonclassical MHC class I antigen. These heterodimers are reported to transduce signals following ligand binding. NKG2A is shown to be inhibitory in nature, while NKG2C and NKG2E transduce stimulatory signals. Comparatively, NKG2A is more abundant than NKG2C and NKG2E in NK cells. Murine NKG2A contains two intracytoplasmic sequences that closely resemble the Immunoreceptor Tyrosine- based Inhibitory Motif (ITIM). N2851-75H can be used for the identification of NK cells expressing functional CD94/NKG2 receptors and is suitable for blocking NKG2A signals in murine tumor models. (Ref.: Vance, RE., et al. (1999). J. Exp. Med. 190(12); 1801-1812). Applications: Suitable for use in Flow Cytometry and Inhibition Assays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug detected NKG2A/C/E in one million C57BL/6 mouse splenocytes. Optimal dilution determined by the researcher Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice