N2851-75

NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c)

Size

£665.00

SKU:: N2851-75
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Spleen and bone marrow cells from CE mice.
Clone:: PK136
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. Clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. This clone has also been used for in vivo depletion of NK cells and in vitro activation of NK cells. IgG Goat anti mouse IgG: RPE (rat adsorbed) Fusion Partners: Spleen cells from immunized (C3HxBALB/c) FI Hybrid were fused with cells of the Sp2/0-Ag14 myeloma cell line. Applications: Immunoprecipitation Flow Cytometry: 5-10ug/ml Complement dependent lysis: 1-10ug/ml Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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