N2850-34

Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

Size

£863.00

SKU:: N2850-34
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Pro120 from human Nkx6.1 expressed in E. coli. Species Seqeuence Homology: mouse, 96%
Clone:: 11D12
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Nkx6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. Nkx6.1 exhibits both transcriptional gene repression (Nkx6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b cell mitosis. Human Nkx6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over (aa1-120), human Nkx6.1 shares 96% aa identity with mouse Nkx6.1. Applications: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml using immersion fixed BTC-6 mouse beta cell insulinoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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