N2850-33
Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)
Size
£908.00
- SKU:
- N2850-33
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Met1-Pro120 of human NKX6.1 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-46kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b-cell mitosis. Human NKX6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over aa1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1. Applications: Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using bTC-6 mouse beta cell insulinoma cell line. Immunohistochemistry (Paraffin): 5-15ug/ml detects NKX6.1 in immersion fixed paraffin-embedded human pancreas. Immunohistochemistry (Frozen): 5-15ug/ml detects NKX6.1 in perfusion fixed frozen sections of rat pancreas. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice