N2750-10B

NIS (Sodium Iodide Symporter, Na(+)/I(-) Symporter, Sodium/iodide Cotransporter, Na(+)/I(-) Cotransporter, Solute Carrier Family 5 Member 5, SLC5A5)

Size

£626.00

SKU:: N2750-10B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mannose binding protein hNIS fusion (aa468-643).
Clone:: 12F4
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
Extra Details:: The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3). Applications: Suitable for use Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot (ECL): 1:1000 detects hNIS in 20ug of transfected COS-7 cell membrane lysate using Goat anti-mouse IgG:HRP Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.