N2700-16D-ML650
Nitrotyrosine (nTyr) (MaxLight 650)
Size
£960.00
- SKU:
- N2700-16D-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Protein-bound Nitrotyrosine
- Clone:
- 10A9 (6B2-3G2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis. Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: FLISA: 2-100ng/ml Western Blot: 0.1ug/ml Immunoprecipitation: 1ug/ml Optimal dilutions to be determined by the researcher. Crossreactivity Data: Specificity as determined by FLISA competition experiments. Compound Inhibition Nitrotyrosine 95% Nitrotryptophan 4% Chlorotyrosine 3% Phosphotyrosine 2% Tyrosine 0% Aminotyrosine 0% Recommended Secondary Antibodies: I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo I1904-65N: IgG (HRP) Pab Rb xMo I1903-07E: IgG (HRP) Pab Rb xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice