N2700-16D-BIOTIN
Nitrotyrosine (nTyr) (Biotin)
Size
£960.00
- SKU:
- N2700-16D-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Protein-bound Nitrotyrosine
- Clone:
- 10A9 (6B2-3G2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 2-100ng/ml Western Blot: 0.1ug/ml Immunoprecipitation: 1ug/ml Optimal dilutions to be determined by the researcher. Crossreactivity Data: Specificity as determined by ELISA competition experiments. Compound Inhibition Nitrotyrosine 95% Nitrotryptophan 4% Chlorotyrosine 3% Phosphotyrosine 2% Tyrosine 0% Aminotyrosine 0% Recommended Secondary Antibodies: I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo I1904-65N: IgG (HRP) Pab Rb xMo I1903-07E: IgG (HRP) Pab Rb xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice