N2700-09-BIOTIN
Nitrotyrosine (nTyr) (Biotin)
Size
£1,106.00
- SKU:
- N2700-09-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Nitrotyrosine conjugated to KLH
- Clone:
- 3G210
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in the presence of the active metabolite NO. Various pathways including the formation of peroxynitrite lead to nitrotyrosine production. It is still unknown whether nitration is merely a footprint of oxidative stress, an important pathway of nitric oxide metabolism or a part of integral processes for maintaining cellular homeostasis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen/Paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice