N2568-01A
NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1, NIPP-1, Protein Phosphatase 1 Regulatory Inhibitor Subunit 8, ARD1, PPP1R8)
Size
£642.00
- SKU:
- N2568-01A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-EAWPGKKPTPSLLI, from human PPP1R8, at C-terminal (NP_002704.1, NP_054829.2, NP_612568.1).
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Immunohistochemistry (Paraffin): 2.5-3.8ug/ml Western Blot: 1-2ug/ml, observed in human lung and H460 cell extracts on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice