Nicotinic Acetylcholine Receptor alpha 1 (nAChR, Acetylcholine Receptor Protein alpha Chain Precursor, ACHRA, ACHRD, Cholinergic Receptor Nicotinic alpha Polypeptide 1, CHRNA1, CHNRA, CMS2A, FCCMS, SCCMS, Schizophrenia Neurophysiologic Defect Candidate)

Size

£767.00

SKU:: N2562-08D
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa22-36, EHETRLVAKLFKDYS from the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1. Species Sequence Homology: mouse.
Format:: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH20.
Extra Details:: Upon binding of acetylcholine, the acetylcholine receptor responds by undergoing an extensive change of conformation which affects its alpha and beta subunits and leads to the opening of an ion-conducting channel across the plasma membrane. At least 2 isoforms of the alpha-1 subunit are produced from alternate splicing. Isoform 1 is only expressed in skeletal muscle whereas isoform 2 is constitutively expressed in skeletal muscle, brain, heart, kidney, liver, lung and thymus. Applications: Suitable for use in Western Blot Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.5ug/ml. Band expected at ~55kD. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice