N2498-04-ML490
Nibrin (Nijmegen Breakage Syndrome, NBS1) (MaxLight 490)
Size
£1,010.00
- SKU:
- N2498-04-ML490
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein corresponding to residues 420-774 of human Nibrin.
- Clone:
- EE15
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunoprecipitation: 4ug immunoprecipitated Nibrin/Nbs1 from 500ug of Jurkat RIPA lysate. Immunocytochemistry: 2ug/ml detected Nibrin/Nbs1 in A431 cells. Western Blot: 1ug/ml detected Nibrin/Nbs1 in 10ug of HeLa cell lysate. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice