N2375-29A
NFATC3 (Nuclear Factor of Activated T-cells, Cytoplasmic, Calcineurin-dependent 3, NFATX)
Size
£800.00
- SKU:
- N2375-29A
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp900-Asp1035 from human NFATC3 expressed in E. coli.
- Clone:
- 11n141
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- NFATC3 is a 145-150kD member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075aa in length. It contains a calcineurin binding site aa109-114, two NLSs aa273-275, 686-688, one NES aa1032-1041 and an RHD that binds DNA aa433-592. There are multiple isoforms. Thirty aa, 33aa and 10aa substitutions for aa1036-1075 are known that may be accompanied by an alternate start site at aa480. Over aa900-1035, human NFATC3 shares 93aa identity with mouse NFATC3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice