N2375-21A

NFAT2 (NFATC1, Nuclear Factor of Activated T-cells)

Size

£927.00

SKU:: N2375-21A
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
Clone:: 6D606 (7A6)
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: The Nuclear Factor of Activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively coordinate the immune response. Two forms of NFAT, the cytosolic (NFATc1) and the pre-existing NFATc2 (formerly NFATp), have been recognized on separate genes, and show sequence similarity with the Dorsal/Rel family of transcription factors. Studies indicate that NFATc is required for interleukin (IL-2) gene expression, is found primarily in lymphoid tissues and is induced upon T-cell activation. NFATc2 shows 73% homology with NFATc1 within the Rel-similarity region, but has a wider tissue distribution and does not appear to be inducible. T-cell activation requires two signals for initiation, one from the antigen receptor and the other by accessory cells or agents which activate protein kinase C. Upon binding of the antigen to the T-cell receptor, several membrane events are initiated which are similar to those initiated by many hormones, including the mobilization of intracellular calcium stores. Applications: Suitable for use in Western Blot, Immunocytochemistry Immunoprecipitation, Immunoflourescence, Immunohistochemistry and Gel Supershift Assay. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry: 1:250 Western Blot: 1:100-1:2000 Immunohistochemistry: 1:20-1:200 Immunocytochemistry/Immunofluorescence: 1:10-1:500 Immunoprecipitation: 1:10-1:500 Chromatin Immunoprecipitation: 1:10-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice