NFATC1 (Nuclear Factor of Activated T-cells, Cytoplasmic 1, NF-ATc1, NFATc1, NFAT Transcription Complex Cytosolic Component, NF-ATc, NFATc, NFAT2, NFATC)

Size

£581.00

SKU:: N2375-10D
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa263-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 (NP_006153).
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: NFAT (nuclear factor of activated T cell transcription factors play critical roles in gene transcription during immune responses. The NFAT complex is made up of NFATc (NFATc1-4) protein and activator protein-1 (AP-1) transcription factor. NFAT proteins are regulated by Ca2+. NFATc1 is a substrate protein phosphatase calcineurin, a critical mediator of calcium signals during T-cell activation. NFATc1 activation requires the translocation of the transcription factor into the nucleus, a process that is mediated by calcineurin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Ramos cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.