N2350-08M
Nerve Growth Factor, beta, Recombinant, Mouse (NGFb)
Size
£448.00
- SKU:
- N2350-08M
- Molecular Weight:
- 26.8
- Purity:
- ≥ 98% (SDS-PAGE gel and HPLC analyses). Endotoxin: ≤ 0.1ng/ug (<IEU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of ≥0.1mg/ml.
- Extra Details:
- β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. The functional form of recombinant mouse β-NGF is a non-covalently-linked homodimer of two 13.4kD, polypeptide monomers that each contain 120 amino acids and three disulfide bonds, which are required for biological activity. Molecular Weight: ~26.8kD Amino Acid Sequence (monomer): SSTHPVFHMG EFSVCDSVSV WVGDKTTATD IKGKEVTVLA EVNINNSVFR QYFFETKCRA SNPVESGCRG IDSKHWNSYC TTTHTFVKAL TTDEKQAAWR FIRIDTACVC VLSRKATRRG Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤1ng/ml, corresponding to a specific activity of ≥1x10e6 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice