N2350-05-ML550

NGFB (Beta-nerve Growth Factor, Beta-NGF, NGF, HSAN5, MGC161426, MGC161428) (MaxLight 550)

Size

£812.00

SKU:: N2350-05-ML550
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human NGFb, expressed in E. coli
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Extra Details:: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Nerve growth factor-beta (NGF-b) is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, formed by two 13.5kD polypeptide monomers (238 total aa). The three disulfide bonds are required for biological activity. Applications: Suitable for use in FLISA, Western Blot and Neutralization. Other applications not tested Recommended Dilution: FLISA: 0.5-2ug/ml. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF as a detection antibody, allows the detection of 0.2-0.4ng/well of recombinant Human beta-NGF. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition of the biological activity of Human beta-NGF (1ng/ml), a concentration of 0.05-0.08ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.

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