NFkB, p100/p52 (Nuclear Factor NF-kappa-B p100 Subunit, DNA-binding Factor KBF2, H2TF1, Lymphocyte Translocation Chromosome 10 Protein, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 2, Oncogene Lyt-10, Lyt10, Nuclear Factor NF-kappa-B p52 Subunit, NFKB2, LYT10)

Size

£735.00

SKU:: N2305-02K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic non-phosphopeptide corresponding to human NFkB p100/p52 around amino acids 870~874 (S-Q-S-V-E).
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:100-1:200 Immunofluorescence 1:100-1:200 Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: NFkB p105/p50 Overexpression Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice