NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B Cells 3, NFKB3, RELA) [KO Validated]

Size

£952.00

SKU:: N2303-10L
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant corresponding to Asn456-Ser551 from human RelA expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: RelA belongs to a family of transcription factors (NFKB) that play a fundamental role in inflammatory and immune responses. The NFKB complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NFKB1 or NFKB2 subunits. RelA and NFKB1 are the most common heterodimeric pair. The NFKB complex is sequestered in the cytoplasm by inhibitory IKB proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the IKB proteins allowing the NFKB complex to translocate to the nucleus and activate gene transcription. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunoprecipitation: 5ug labels 5 x10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice