NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA)

Size

£639.00

SKU:: N2303-02A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A portion of the NF-kB p65 nuclear localization signal (DTDDRHRIEEKRKRKT).
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Extra Details:: P65 NLS is a transcription factor belonging to NF-kappa B family with the N-terminal Rel homology domain of the NF-kB family, a C-terminal transcription activation domain and a nuclear receptor interaction motif (LXXLL). p65 is a part of the nuclear localization signal (NLS) polypeptide of NF-kB (p50/p65) heterodimer. IkBa the most abundant IkB interacts with active NF-kB p65 homodimers and inactivates their transcriptional potential by trapping them in the cytoplasm and through stable complex formation. It is ubiquitously expressed in most of the tissues. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunocytochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.