N2303-01A
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Size
£704.00
- SKU:
- N2303-01A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
- Clone:
- 4H211 (112A1021)
- Format:
- Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
- Extra Details:
- NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 25ug/ml Flow Cytometry: 0.25-0.5ug/10e6 cells Immunohistochemistry (Frozen): 5-15ug/ml Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: NFkB p105/p50 Overexpression Lysate (Native) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice