N2302-04
NFkB, p65, phosphorylated (Ser536) (NF-kB, Nuclear Factor kappa B, RelA)
Size
£639.00
- SKU:
- N2302-04
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65. Species sequence homology: canine and porcine
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- The transcriptional nuclear factor kappaB (NF-kappaB) plays a pivotal role in inflammatory and immune responses (1,2). NF-kappaB is composed of a heterodimer of p65 and p50 subunits in most cell types and is sequestered in the cytoplasm by its inhibitory proteins, the IkappaBs (3-5). NF-kappaB-activating agents can induce the phosphorylation of IkappaBs; the phosphorylation targets them for rapid degradation through a ubiquitin-proteasome pathway, thereby releasing NF-kappaB to enter the nucleus for gene expression (6-8). It has been shown that the p65 NF-kappaB subunit is also phosphorylated during the phosphorylation and degradation of IkappaBs; the phosphorylation site is Ser536 in the C-terminal transactivation domain (9). Applications: Suitable for use in ELISA and Western Blot. Others not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice