N2302-03
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Size
£793.00
- SKU:
- N2302-03
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peptide corresponding to human p65 coupled to BSA.
- Clone:
- 2Q2254 (12H11)
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- The nuclear factor kB (NFkB) is a sequence-specific DNA-binding protein (2). It is a pleiotropic transcription factor which is involved in the expression of a variety of cellular and viral genes (3). NFkB consists of two subunits which are named according to their molecular weight, p50 and p65 (4). The subunits are stabilized by a so-called inhibitory chain IkB (5). In quiescent cells, NFkB resides in the cytosol in an inactive form which can be activated in vivo by treatment of cells with cytokines or protein kinase activators (6). In vitro, it is possible to generate the active form of NFkB by treatment with sodium deoxycholate, formadine or electrophoretic size fractionation. The active form of NFkB is a heterotetrameric protein, consisting of the two p50 and two p65 subunits (7). After activation NFkB translocates from the cytosol to the nucleus of the cell, binds to specific DNA sequences and initiates transcription. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Electrophoretic mobility supershift assays (EMSA), and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1-10ug/ml Western Blot: 1:500 Immunohistochemistry (paraffin): 5-10ug/ml, APAAP Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunohistochemistry (frozen): 5-10ug/ml, APAAP Acetone or methanol fixed cells Supershift assay: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4°C Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice