N2302-03-ML650
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) (MaxLight 650)
Size
£1,049.00
- SKU:
- N2302-03-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Peptide corresponding to human p65 coupled to BSA.
- Clone:
- 2Q2254 (12H11)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The nuclear factor kB (NFkB) is a sequence-specific DNA-binding protein (2). It is a pleiotropic transcription factor which is involved in the expression of a variety of cellular and viral genes (3). NFkB consists of two subunits which are named according to their molecular weight, p50 and p65 (4). The subunits are stabilized by a so-called inhibitory chain IkB (5). In quiescent cells, NFkB resides in the cytosol in an inactive form which can be activated in vivo by treatment of cells with cytokines or protein kinase activators (6). In vitro, it is possible to generate the active form of NFkB by treatment with sodium deoxycholate, formadine or electrophoretic size fractionation. The active form of NFkB is a heterotetrameric protein, consisting of the two p50 and two p65 subunits (7). After activation NFkB translocates from the cytosol to the nucleus of the cell, binds to specific DNA sequences and initiates transcription. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Gel Supershift, Electrophoretic mobility supershift assays (EMSA), Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): APAAP Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunohistochemistry (frozen): APAAP Acetone or methanol fixed cells Optimal dilutions to be determined by the researche Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice