N2301-51F
NFkB, p50 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subunit)
Size
£320.00
- SKU:
- N2301-51F
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to full-length human NF-kB p50.
- Clone:
- 4D1
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- NF-kB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-kB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65kD transactivating subunit and a 50kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-kB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-kB/p50 is bound (with p65) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB which goes to the nucleus to activate gene expression. NF-kB can be activated with LPS, TNF-a, phorbol ester, or IL-1. Applications: Suitable for use in Immunocytochemistry and Western Blot. Not recommended for ChIP (Chromatin Immunoprecipitation). Other applications not tested. Recommended Dilution: Western Blot: 0.025-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice