N2301-39B

NFkB2 (Nuclear Factor kappa B)

Size

£863.00

SKU:: N2301-39B
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Met1-Asn447 of human NFkB2, expressed in E. coli.
Clone:: 7H260
Format:: Supplied as a powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Nuclear Factor kappa B2 (NFκB2 or NFκB p52) is a member of the NFκB/Rel family of transcription factors. NFκB2 dimerizes with other members of the NFκB/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes. Applications: Suitable for use in ChIP, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Chromatin Immunoprecipitation (ChIP): 5ug per 5x10e6 cells Immunocytochemistry: 3-25ug/ml using immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.