N2205
Neurturin (NTN)
Size
£655.00
- SKU:
- N2205
- Application:
- WB
- Purity:
- Immunoaffinity purified IgG
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to amino acids 178 to 193 of human neurturin (1).
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin (1, 2). Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRa (3,4). The cDNAs encoding NTNRa from human, rat, chicken, and mouse have been cloned recently (3-8) and termed GDNFRb, Ret ligand 2 (RETL2) or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently (9). NTN binds to and forms a complex with GFRa-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000. An approximate 14kD band of the full length recombinant NTN was detected. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%) aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice