Neurotensin Receptor 3 (NTR3, Sortilin)

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£754.00

SKU:: N2177-52
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide sequence corresponding to 18aa close to the extracellular, N-terminus region of human NTR3 conjugated to KLH. Species Sequence Homology: mouse and rat NTR3: 94%
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals. Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion. NT is widely distributed throughout the CNS. It has been localized to catecholamine-containing neurons. NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13). Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1. Most recently, a novel, non-GPCR, NTR3/sortilin/gp95/NT3 (mouse 825 aa, rat 748 aa; human 833 aa, chromosome 1p21.3; ~92% identity between human and mouse) also binds NT. Sortilin was initially isolated as by its affinity for receptor-associated protein (RAP). NTR3, the first non-GPCR neuropeptides receptor, sequence includes a putative secretory signal peptide, a furin cleavage site, and a single transmembrane domain near the C-terminus. The mature protein has 44 N-terminal residues removed during processing. It is homologous to the yeast protein Vps10p and the human sortilin-related receptor. The C terminus of the protein contains a lysosomal sorting motif shared by the mannose-6-phosphate receptors. NTR3 is expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis. Applications: Suitable for use in ELISA. Other potential application, though not tested is Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: N2177-50: Neurotensin Receptor 3, Control Peptide, Human (NTR3, Sortilin) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice