N2173-20
Neuron Specific Nuclear Protein (NeuN) (Biotin)
Size
£877.00
- SKU:
- N2173-20
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified cell nuclei from mouse brain. Species Sequence Homology: human, ferret, chick and salamander
- Clone:
- 3H412 (A60)
- Format:
- Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:100 using rat cerebellum; tissue pretreated with citrate buffer, pH 6. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% Triton X-100. Optimal dilutions to be determined by the researcher. Recommended Control: Brain tissue, most neuronal cell types throughout the adult nervous system Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice