N2160-06B
Neurofilament heavy polypeptide (NF-200, NF200, NF-H, NEFH, N52, Neurofilament triplet H protein, 200kD neurofilament protein, KIAA0845, NFH)
Size
£512.00
- SKU:
- N2160-06B
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- C-terminal segment of enzymatically dephosphorylated porcine Neurofilament 200.
- Clone:
- 10K289
- Format:
- Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 Kd (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1-2ug/ml Immunohistochemistry (Frozen): 1-2ug/ml using formalin or acetone fixed tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice