N2159-50

Neurofilament M 160-200kD (NF-M)

Size

£787.00

SKU:: N2159-50
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat NF-M
Clone:: 3G201
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Neurofilaments (NFs) are prominent components of large myelinated axons. Based on sequence homology and intron placement, the NF genes have been classified as type IV IFs along with alpha-internexin, which is also expressed in the nervous system. NFs may function as effector proteins subject to a complex regulatory cross-talk between neurons and myelinating Schwann cells or oligrodendrocytes. Mammalian neurofilaments are assembled from light (NF-L), mid-sized (NF-M), and heavy (NF-H) neurofilament proteins. NF-M is required if myelinated axons are to achieve maximal diameters and NF-M has been shown to regulate the level of NF-L. NF-M subunits mainly regulate axonal caliber by altering the level of NF-L and in turn the number of NFs in the axon. Results seem to explain as a perturbation of NF stoichiometry in which overexpression of NF-M leads to the formation of perikaryal NF inclusions in motor neurons and the depletion of NFs from axons. Overexpression of NF-M in combination with NF-L increased radial growth of axons, which suggests that NF-M or NF-H must coassemble into filaments with NF-L before they can affect the radial growth of axons. The immature form of NFs composed of NF-L and NF-M, might function in establishing the early neuronal phenotype and in maintaining neurite outgrowth. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:50-1:100 Western Blot: 0.5-2ug/ml Immunohistochemistry (FFPE and Frozen Sections): 1:20-1:1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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