NFBD1 (Nuclear Factor with BRCT Domains Protein 1, MDC1, KIAA0170)

Size

£537.00

SKU:: N2159-25
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acids 871-888 of human NFBD1.
Format:: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Extra Details:: NFBD1, nuclear factor with BRCT domains protein 1, participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) superfamily of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence. A more recent renaming of NFBD1 is MDC1 (mediator of DNA damage checkpoint protein). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:1000 observed size: ~220 kD positive control: Jurkat whole cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.