N2146-25A

Neuroendocrine Specific Protein C (NSP-C, Rtn 1C, Reticulon 1C)

Size

£659.00

SKU:: N2146-25A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Neuroendocrine Specific Protein C
Clone:: 4A71
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Extra Details:: Recently, a novel gene family has been identified and characterized, the protein products of which are localized in the endoplasmic reticulum. The proteins encoded by these genes were therefore named Reticulons. Reticulon-1 (Rtn-1) was formerly designated NSP (Neuroendocrine-Specific-Protein), because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. NSP-A, NSP-B, and NSP-C. These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. NSP-A is a protein with a molecular weight (MW) of about 135kD, which occurs in various isoforms presumably depending on the degree of phosphorylation in serine residues. In lung cancer diagnosis NSP-A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of NSP-A. NSP-B is a phosphoprotein with a MW of 45kD and is restricted to the lung cancer cell line NCI-H82. NSP-B is not found for so far in human tissues. NSP-C is a protein with a MW of 23kD which is not phosphorylated and is found with NSP-A in SCLC (cell lines) and not in non-SCLC (cell cultures). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100 Immunohistochemistry: 1:50-1:100; Frozen tissue sections with avidin-biotinylated horseradish peroxidase complex (ABC) as the detection reagent. Western Blot: 1:100-1:500 Optimum dilutions to be determined by researcher. Hybridoma: Derived by the fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a BALB/c mouse. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice