N2143-30C
NeuroD2 (Neurogenic Differentiation Factor 2, NDR2, KW8, NeuroD-related Factor, NDRF, NRDL, NeuroD Family Helix-loop-helix Transcription Factor)
Size
£863.00
- SKU:
- N2143-30C
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to aa174-382 from human NeuroD2 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- NeuroD2 is a 41kD nuclear member of the neuroD family of transcription factors. It is expressed in developing and mature neurons such as hippocampal granular neurons, and acts (in part) to repress factors that would otherwise block multipotential cell commitment to a neuronal lineage. NeuroD2 is presumed to act as a heterodimer with other bHLH transcription factors. Human NeuroD2 is 382aa in length. It contains a poly-Glu region aa82-91, an NLS aa107-113, a DNA-binding HLH domain aa119-178 and a poly-Gly segment aa282-285. Based on rat studies, human NeuroD2 will undergo variable phosphorylation in the C-terminal region. Over aa174-382, human and mouse NeuroD2 possess identical aa sequences. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice