Neurite Outgrowth Inhibitor A, NT (NogoA, NOGO-A, ASY, Foocen, KIAA0886, My043, Neurite Outgrowth Inhibitor, NOGO, Nogo Protein, NOGOC, Nogo-C, Neuroendocrine-specific Protein, NSP, Neuroendocrine-specific Protein C Homolog, NSP-CL, NI220/250, Reticulon 4, Reticulon-4, RTN4, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, Reticulon-5, SP1507) (HRP)

Size

£902.00

SKU:: N2115-10N-HRP
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a 23aa sequence from near the N-terminus of human NogoA. Epitope: within the first 50aa
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: detects a band of ~180kD in mouse brain tissue lysate. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended. Immunohistochemistry: 20ug/ml. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Mouse brain Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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