N2110-04
Neurexin 1b
Size
£843.00
- SKU:
- N2110-04
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- NS0-derived recombinant rat Neurexin 1Beta, Ala47-Thr392
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Neurexin 1 beta is an 80kD member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an a- (long) and B- (short) isoform. Mature rat Neurexin 1B is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47-392) that contains one laminin-like G domain (aa 91-264). Within the G domain, the presence or absence of a splice site (aa 201-230) materially impacts neuroligin binding. Over aa 47-392, rat Neurexin 1B is 100% and 98% aa identical to mouse and human Neurexin 1B, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using rat spinal cord tissue as a sample. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice