N2100

Neuraminidase, Clostridium perfringens (Sialidase, nanH, NA)

Size

£403.00

SKU:: N2100
Purity:: Chromatographically purified.
Storage Conditions:: 4°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 50% sucrose
Extra Details:: Neuraminidase (sialidase) splits off N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins and are important tools for the study of glycoproteins in protein chemistry and cell biology (Hatton et al. 1973). Chromatographic purification has been developed in this laboratory based on work of Cassidy et al. (1965). Wooley and Gommi (1966) describe the use of this neuraminidase in a method for measuring serotonin receptors. It is also employed to determine bound N-acetylneuraminic acid in tissues. Considerable interest has been shown in using neuraminidase inhibitors as possible anti-viral and anti-bacterial agents. Form: Supplied as a lyophilized powder, 50% sucrose Concentration: ~20u/mg Protein Content (%): ≥30% Units/Protein (u/mg): ≥5u/mg Protein (dry weight) A280@mg/ml: ~0.4 Assay Method: The assay is based on the measurement of sialic acid (NANA) released from bovine submaxillary mucin. One unit causes the release of one micromole of sialic acid per minute at 37°C and pH 5.0, from bovine submaxillary mucin under the specified conditions. Storage and Stability: Product is stable for 6 months after receipt at 4°C.