NHE7 (Na, H Exchanger 7)

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£754.00

SKU:: N2021-50
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa from human NHE7 at N-terminal, conjugated to KLH. Species sequence homology: chimp;100%, mouse and rat; 94%. Sequence homology: NHE6; 70%, but relatively less with other NHEs; 25% .
Format:: Supplied as a lyophilized powder from PBS, pH 7.5, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70% related to NHE6 but relatively less (~25%) homolohgous with other NHEs. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 1:100,000 using 50-100ng control peptide/well. Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissues. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: N2021-75: NHE7, Human (Na, H Exchanger 7) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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