N2017-41

Neurotrophin Receptor, p75

Size

£1,010.00

SKU:: N2017-41
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human melanoma cell line WM245
Clone:: ME20.4
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Extra Details:: Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml of this lot detected p75NTR in RIPA lysates from HS294T cells. Immunohistochemistry: Reported to detect p75NTR in tissue section. Also used in immuno-electron microscopy. Biological Blockade: Reported to block 125I-labeled NGF binding to its receptor. Immunoprecipitation: 4ug of a previous lot immunoprecipitated p75NTR from 500ug of HS294T RIPA lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.