N2017-30
NHE3 (Na, H Exchanger 3)
Size
£754.00
- SKU:
- N2017-30
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated synthetic peptide consisting of 22aa within the cytoplasmic, C-terminal domain of the rat NHE3. Species Sequence Homology: Mouse: 90%; Human: 76%.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
- Extra Details:
- Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to transcellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE3 (rat, 831aa; human chromosome 5p15.3) is involved in trans-epithelial sodium absorption. The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 (using 50-100ng control peptide/well) Optimal dilutions to be determined by the researcher. Control Peptide: N2017-31 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice