NHE3 (Na, H Exchanger 3) (Not for Export EU)

Size

£704.00

SKU:: N2017-25
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: KLH-conjugated synthetic peptide consisting of 22aa within the cytoplasmic, c-terminal domain of the rat NHE3. Species Sequence Homology: Mouse: 90%; Human: 76%.
Format:: Supplied as a liquid.
Extra Details:: Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Seven NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. NHE3 (rat, 831aa; human chromosome 5 p15.3) is involved in trans-epithelial Na+ absorption. The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach (1). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: N2017-29 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice