NHE1 (Na, H Exchanger 1)

Size

£754.00

SKU:: N2015-07
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Synthetic peptide corresponding to 22a within the cytoplasmic, c-terminal domain of the rat NHE1, conjugated to KLH. Species Sequence Homology: human, mouse, bovine, porcine and rabbit; 100%; hamster; 90%.
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1-10ug/ml using ECL. A band of 95-110kD has been detected in brush border membrane. ELISA: 1:100,000; using 50-100ng control peptide per well. Optimal dilutions to be determined by the researcher. Control Peptide: N2015-09: NHE1, Rat (Na, H Exchanger 1) (Control Peptide) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice