N2015-03
NHE1 (Na, H Exchanger 1, Slc9a1)
Size
£780.00
- SKU:
- N2015-03
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 22aa, within the cytoplasmic, C-terminal domain of rat NHE1 (KLH).
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
- Extra Details:
- Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of Na+ and H+ ions in various tissues. The NHE catalyzes the electoneuronal transport of extracellular Na+ for intracellular H+. They play a major role in the regulation of intracellular pH (pHi), addition to trans-cellular absorption of Na+, cell volume regulation, and possibly cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer, and renal intestinal disorders. Five isoforms (NHE1–5) have been cloned so far. They are all similar in their primary structure and predicted to have 10–12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular. NHE1 is “House Keeper” and is expressed ubiquitously in all tissues. It is implicated in pH homeostasis, volume regulation, and probably cell proliferation. It is expressed on the basolateral surface of several epithelia (1). Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-10ug/ml (ECL). ELISA: 1:100,000. Using 50-100ng control peptide per well. Optimal dilutions to be determined by the researcher. Control Peptide: N2015-05: NHE1, Control Peptide, Rat (Na, H Exchanger 1) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice