N1263
Neurolysin (Neurolysin, Mitochondrial, Angiotensin-binding Protein, Microsomal Endopeptidase, MEP, Mitochondrial Oligopeptidase M, Neurotensin Endopeptidase, NLN, AGTBP, KIAA1226)
Size
£819.00
- SKU:
- N1263
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Purified, E. coli-derived, recombinant human Neurolysin (aa38-704).
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- NLN (neurolysin) is a metalloproteinase of the MA(E) clade, in the M3 family. The name is derived from the ability to degrade neurotensin. The M3 family includes the structurally similar thimet oligopeptidase (EC 3.4.24.15), and contains the canonical HExxHxxH zinc binding motif in the catalytic domain, but with the third histidine three residues closer in than the MMP family. Neurolysin has been reported in the cytosol and in mitochondria, and in pig, rat and chimp there are alternatively spliced versions that target neurolysin to the mitochondria. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml with lysates of SH-SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice