N1260-03D1

NEUROLIGIN 3 (NLGN3, ASPGX1, AUTSX1, Gliotactin Homolog, HNL3, KIAA1480, Neuroligin-3, NL3)

Size

£825.00

SKU:: N1260-03D1
Application:: WB
Purity:: Purified by Protein A/G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gln38-Ser709 from human Neuroligin 3/NLGN3, expressed in NSO cells. Species sequence homology: Recombinant human Neuroligin 3v2 and recombinant rat Neuroligin 3v4 (100%).
Clone:: 11n136
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114kD member of the type-B carboxyesterase/lipase family of proteins. It is a neuronal transmembrane protein that forms Ca++-dependent intercellular junctions with short beta -neurexin isoforms. This seems to contribute to both glutamatergic and GABAergic synapse formation. Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism. Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein. It contains a 672aa extracellular domain (ECD) (aa38-709), plus a 118aa cytoplasmic region. The ECD possesses a nonfunctional carboxyesterase domain (aa41-625). Multiple splice variants exist. There is a deletion of aa153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa153-192 that may also be accompanied by a five Lys substitution for aa554-848. Over aa39-709, human NLGN3 shares 99% aa identity with mouse NLGN3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice