Neuroligin 2 (NLGN2, KIAA1366)

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£856.00

SKU:: N1260-02B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gln15-Ser660 of human Neuroligin 2 expressed in NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: NLGN2 (Neuroligin 2) is a 105kD member of the type-B carboxylesterase family of enzymes. It is expressed at GABAergic synapses, and is assumed to play both an organizing and regulatory role in inhibitory synapse signaling. It is suggested to bind to beta -neurexin-1, 2 and 3 as a dimer in trans, and to interact cytoplasmically with PDZ-domain containing proteins. Mature human NLGN2 is an 821aa type I transmembrane (TM) glycoprotein. It contains a nonfunctional type-B lipase domain (aa40-601), a TM segment (aa678-698), and a cytoplasmic tail with a PZD-binding region (aa785-835). There are potential splice forms. Individually, or in combination, there is an alternate start site at Met512, a deletion of aa153-169, and a 99aa substitution for aa 806-835. Over aa1-677, human NLGN2 shares 98% aa identity with mouse NLGN2. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-3 ug/ml using human cortex and rat cerebellum tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice